Modeling Neurospora growth

Modeling Neurospora growt

Integrated three-dimensional models for noninvasive monitoring and valorization of the Morgantina silver treasure (Sicily)

The Morgantina silver treasure belonging to the Archaeological Museum of Aidone (Sicily) was involved in a three-dimensional (3-D) survey and diagnostics campaign for monitoring the collection over time in anticipation of their temporary transfer to the Metropolitan Museum of Art in New York for a period of 4 years. Using a multidisciplinary approach, a scientific and methodological protocol based on noninvasive techniques to achieve a complete and integrated knowledge of the precious items and their conservation state, as well as to increase their valorization, has been developed. All acquired data, i.e., 3-D models, ultraviolet fluorescence, x-ray images, and chemical information, will be made available, in an integrated way, within a web-oriented platform, which will present an in-progress tool to deepen existing archaeological knowledge and production technologies and to obtain referenced information of the conservation state before and after moving of the collection from its exposure site

Analysis of the Secondary Structure of the Catalytic Domain of Mouse Ras Exchange Factor CDC25Mm

The minimal active domain GEF domain. of the mouse Ras exchange factor CDC25Mm was purified to homogeneity from recombinant Escherichia coli culture. The 256 amino acids polypeptide shows high activity in vitro and forms a stable complex with H-ras p21 in absence of guanine nucleotides. Circular dichroism CD. spectra in the far UV region indicate that this domain is highly structured with a high content of a-helix 42%.. Near UV CD spectra evidenced good signal due to phenylalanine and tyrosine while a poor contribution was elicited by the three tryptophan residues contained in this domain. The tryptophan fluorescence signal was scarcely affected by denaturation of the protein or by formation of the binary complex with H-ras p21, suggesting that the Trp residues, which are well conserved in the GEF domain of several Ras-exchange factors, were exposed to the surface of the protein and they are not most probably directly involved in the interaction with Ras proteins. q1998 Elsevier Science B.

A cell sizer network involving Cln3 and Far1 controls entrance into S phase in the mitotic cycle of budding yeast

Saccharomyces cerevisiae must reach a carbon source-modulated critical cell size, protein content per cell at the onset of DNA replication (Ps), in order to enter S phase. Cells grown in glucose are larger than cells grown in ethanol. Here, we show that an increased level of the cyclin-dependent inhibitor Far1 increases cell size, whereas far1Δ cells start bud emergence and DNA replication at a smaller size than wild type. Cln3Δ, far1Δ, and strains overexpressing Far1 do not delay budding during an ethanol glucose shift-up as wild type does. Together, these findings indicate that Cln3 has to overcome Far1 to trigger Cln–Cdc28 activation, which then turns on SBF- and MBF-dependent transcription. We show that a second threshold is required together with the Cln3/Far1 threshold for carbon source modulation of Ps. A new molecular network accounting for the setting of Ps is proposed

Atti del Workshop: Tecniche Speciali e Avanzate di Dosimetria e Radioprotezione

Atti del Workshop organizzato dall'Associazione Italiana di Radioprotezione (AIRP) in collaborazione con l'Università degli Studi di Palermo, dal titolo "Tecniche Speciali e Avanzate in Dosimetria e Radioprotezione" che si svolgerà venerdì 24 Giugno a Palermo presso l'Aula Magna del Dipartimento di Fisica e Chimica, Viale delle Scienze Edificio 18. L'evento rientra tra i 210 Eventi organizzati per i 210 anni dalla fondazione dell'Ateneo palermitano su iniziativa del Magnifico Rettore, Prof. Fabrizio Micari. L’evento prevede la discussione di 4 tematiche in ambito della dosimetria e della radioprotezione in campo sanitario ed ambientale (Dosimetria Clinica, Dosimetria Ambientale, Dosimetria Retrospettiva e Tecniche Dosimetriche avanzate in Radioprotezione) ciascuna delle quali ha una relazione generale ad invito e alcune brevi comunicazioni orali. Le sessioni sono moderate da Dirigenti Fisici medici di due delle più importanti Aziende Ospedaliere Siciliane, Dirigenti dell’Istituto Superiore di Sanità e l’ex Rettore dell’Ateneo e membro del CdA del CNR, il Prof. Roberto Lagalla. Interverranno il magnifico Rettore dell’Università di Palermo, l’Assessore alla Sanità della Regione Sicilia, il Direttore dell’Istituto Zooprofilattico Sperimentale di Sicilia, il Direttore Generale dell’Agenzia per la Protezione dell’Ambiente Sicilia, il Direttore del Dipartimento di Fisica e Chimica, il Direttore di ATeN Center (Advanced Technologies Network), Dirigenti Fisici dell’Istituto Superiore di Sanità e dell’Istituto Europeo Oncologico di Milano. Hanno contribuito alla parte organizzativa e scientifica Dirigenti dell’ARPA Sicilia, Dirigenti dell’ARNAS Civico di Palermo, Docenti dell’Università di Palermo e di Milano, Ricercatori del CNR e Specializzandi UniPa. Questo evento è di grande rilevanza nazionale in termini di divulgazione scientifica vista la presenza dei relatori e moderatori invitati che ne danno lustro e rilevanza. Sarà un'occasione di confronto e di analisi retrospettiva a quarant'anni della giornata di studio “Problemi e prospettive della Fisica Sanitaria nel settore medico” organizzata dalla Prof.ssa Maria Brai

Archaeometric Study of Two Tanagra Type Statuettes of Unknown Provenance to Support Forensic Study

This paper is concerned with a morphological-stylistic and archaeometric study of two small pottery statues, confiscated by the Cosenza Carabinieri Unit for the Protection of Cultural Heritage and Anti-Counterfeiting (Calabria, Italy). The research aimed to establish the authenticity of the artworks and to verify a possible origin from the same workshop manufacturing, by providing indications about the textural features and raw materials used for their production. For these purposes, the analytical approach involved the use of minero-petrographic and physical analysis, as follows: petrographic analysis (OM), X-ray diffraction (XRD) and thermoluminescence tests (TL). The preliminary observation, which highlights differences in the stylistic features of the two statuettes as well as in the color, morphology and distribution of the white-greyish patina, is further confirmed by the TL investigations. The TL test revealed an ancient production only for one of the analyzed finds and the investigations on the raw materials allowed to relate this to a possible local historical-artistic context. The second statuette, on the other hand, is attributable to a modern production as confirmed by TL measurement

Comparative analysis for the assessment of restoration treatments on stone material from the roman theater of Merida (Spain)

The overall goal of the project is the study of effects of conservation treatments applied on stone material from archaeological sites, i n terms of superficial changes, effectiveness and durability. In this sense, one of the first premises is characterize the surface of the treated and untreated material in order to determine changes in physical and chemical properties